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Addgene

pML5
(Plasmid #203339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLW555
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7567

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    phlF
  • Insert Size (bp)
    602
  • Promoter PTac and PHlyIIR

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    hlyIIR
  • Insert Size (bp)
    636
  • Promoter PPhlF and PTet

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML5 was a gift from Lauren Andrews (Addgene plasmid # 203339 ; http://n2t.net/addgene:203339 ; RRID:Addgene_203339)

  • For your References section:

    Algorithmic Programming of Sequential Logic and Genetic Circuits for Recording Biochemical Concentration in a Probiotic Bacterium. Lebovich M, Zeng M, Andrews LB. ACS Synth Biol. 2023 Aug 15. doi: 10.1021/acssynbio.3c00232. 10.1021/acssynbio.3c00232 PubMed 37581922
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