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pET28-His8-aCat(660-885)
(Plasmid #203334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTNNA1
  • Alt name
    CTNA1-catenin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    750
  • Mutation
    aa 660-885 only
  • GenBank ID
  • Entrez Gene
    CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
  • Tag / Fusion Protein
    • 8xHis with precision protease cleavage site (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His8-aCat(660-885) was a gift from Tina Izard (Addgene plasmid # 203334 ; http://n2t.net/addgene:203334 ; RRID:Addgene_203334)

  • For your References section:

    The nematode alpha-catenin ortholog, HMP1, has an extended alpha-helix when bound to actin filaments. Rangarajan ES, Smith EW, Izard T. J Biol Chem. 2023 Feb;299(2):102817. doi: 10.1016/j.jbc.2022.102817. Epub 2022 Dec 17. 10.1016/j.jbc.2022.102817 PubMed 36539037
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