pET24-aCatenin(82-906)-His6
(Plasmid #203332)

• Purpose: Bacterial expression of wild-type alpha-catenin
• Depositing Lab: Tina Izard
• Publication: Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9. doi: 10.1074/jbc.M112.351023. Epub 2012 Apr 6.

Backbone

• Vector backbone: pET24
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 8000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTNNA1
• Alt name: CTNA1-catenin
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: aa 82-906
• GenBank ID:
• Entrez Gene: CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry