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pET24-aCatenin(82-906)-His6
(Plasmid #203332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 8000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTNNA1
  • Alt name
    CTNA1-catenin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    aa 82-906
  • GenBank ID
  • Entrez Gene
    CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24-aCatenin(82-906)-His6 was a gift from Tina Izard (Addgene plasmid # 203332 ; http://n2t.net/addgene:203332 ; RRID:Addgene_203332)

  • For your References section:

    The cytoskeletal protein alpha-catenin unfurls upon binding to vinculin. Rangarajan ES, Izard T. J Biol Chem. 2012 May 25;287(22):18492-9. doi: 10.1074/jbc.M112.351023. Epub 2012 Apr 6. 10.1074/jbc.M112.351023 PubMed 22493458
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