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pWR54
(Plasmid #203330)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWR9
  • Backbone manufacturer
    Robertson
  • Backbone size w/o insert (bp) 8155
  • Total vector size (bp) 8299
  • Modifications to backbone
    The TDH3 promoter of pWR9 was removed and replaced with the CCW12 promoter to make pWR54.
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TEF1 promoter (S. stipitis)
  • Species
    S. stipitis (yeast)
  • Insert Size (bp)
    726
  • Entrez Gene
    TEF1 (a.k.a. PICST_65303)

Gene/Insert 2

  • Gene/Insert name
    coHPH
  • Species
    Synthetic
  • Insert Size (bp)
    1029

Gene/Insert 3

  • Gene/Insert name
    ACT1 terminator (S. stipitis)
  • Species
    S. stipitis (yeast)
  • Insert Size (bp)
    400

Gene/Insert 4

  • Gene/Insert name
    CCW12 promoter (S. stipitis)
  • Species
    S. stipitis (yeast)
  • Insert Size (bp)
    749

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CACAAAGAACCAACAAACTATG
  • 3′ sequencing primer CTAGTTTGAGTTAAGAAGTAGAGTG
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    coCBG
  • Species
    Synthetic
  • Insert Size (bp)
    1629

Gene/Insert 6

  • Gene/Insert name
    ADH2 terminator (S. stipitis)
  • Species
    S. stipitis (yeast)
  • Insert Size (bp)
    374

Gene/Insert 7

  • Gene/Insert name
    ARS from S. stipitis chromosome 1
  • Species
    S. stipitis (yeast)
  • Insert Size (bp)
    1148

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    coCBG is a codon optimized version of Promega's CBG99 which was synthesized by GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWR54 was a gift from J. Brian Robertson (Addgene plasmid # 203330 ; http://n2t.net/addgene:203330 ; RRID:Addgene_203330)

  • For your References section:

    BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset for repeatedly modifying wild-type Scheffersomyces stipitis. Reichard WD, Smith SE, Robertson JB. mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. Epub 2023 Jun 22. 10.1128/msphere.00224-23 PubMed 37345937
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