evoFERNY-Cas9NG
(Plasmid
#203329)
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PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NG
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepD881-SR
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Backbone manufacturerAtum
- Total vector size (bp) 7659
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsBL21 Star DE3 competent cells and rhamnose induction for protein purification
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameevoFERNY-Cas9NG
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SpeciesSynthetic
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byModified from the David Liu lab (Broad Institute)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
evoFERNY-Cas9NG was a gift from Vijay Sankaran (Addgene plasmid # 203329 ; http://n2t.net/addgene:203329 ; RRID:Addgene_203329) -
For your References section:
Massively parallel base editing to map variant effects in human hematopoiesis. Martin-Rufino JD, Castano N, Pang M, Grody EI, Joubran S, Caulier A, Wahlster L, Li T, Qiu X, Riera-Escandell AM, Newby GA, Al'Khafaji A, Chaudhary S, Black S, Weng C, Munson G, Liu DR, Wlodarski MW, Sims K, Oakley JH, Fasano RM, Xavier RJ, Lander ES, Klein DE, Sankaran VG. Cell. 2023 Apr 27:S0092-8674(23)00332-X. doi: 10.1016/j.cell.2023.03.035. 10.1016/j.cell.2023.03.035 PubMed 37137305
