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Addgene

evoFERNY-Cas9NG
(Plasmid #203329)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203329 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD881-SR
  • Backbone manufacturer
    Atum
  • Total vector size (bp) 7659
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    BL21 Star DE3 competent cells and rhamnose induction for protein purification
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    evoFERNY-Cas9NG
  • Species
    Synthetic
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Modified from the David Liu lab (Broad Institute)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    evoFERNY-Cas9NG was a gift from Vijay Sankaran (Addgene plasmid # 203329 ; http://n2t.net/addgene:203329 ; RRID:Addgene_203329)

  • For your References section:

    Massively parallel base editing to map variant effects in human hematopoiesis. Martin-Rufino JD, Castano N, Pang M, Grody EI, Joubran S, Caulier A, Wahlster L, Li T, Qiu X, Riera-Escandell AM, Newby GA, Al'Khafaji A, Chaudhary S, Black S, Weng C, Munson G, Liu DR, Wlodarski MW, Sims K, Oakley JH, Fasano RM, Xavier RJ, Lander ES, Klein DE, Sankaran VG. Cell. 2023 Apr 27:S0092-8674(23)00332-X. doi: 10.1016/j.cell.2023.03.035. 10.1016/j.cell.2023.03.035 PubMed 37137305
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