pCAGGS-AcGFP-C
(Plasmid #203325)

• Purpose: (Empty Backbone) Express N-terminal AcGFP-fused protein in mammalian cell
• Depositing Lab: Chiaki Murakami
• Publication: Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002/2211-5463.13628.

Backbone

• Vector backbone: pCAGGS
• Backbone manufacturer: Chiaki Murakami
• Backbone size (bp): 5512
• Modifications to backbone: Kozak Sequence (GCCACCATGG), AcGFP, and MCS were inserted via in-Fusion cloning
• Vector type: Mammalian Expression
• Promoter: CAG
• Tag / Fusion Protein:
  • AcGFP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: TTCGGCTTCTGGCGTGTGACC
• 3′ sequencing primer: TATTAGCCAGAAGTCAGATGCTCAAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original plasmid is created by Niwa et al. (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991).