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pOET3-N-TEV-Twin-Strep
(Plasmid #203323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOET3
  • Backbone manufacturer
    Oxford Expression Technology
  • Backbone size (bp) 4640
  • Modifications to backbone
    Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tag (ENLYFQGS- WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) was cloned into the XhoI/BglII site.
  • Vector type
    Insect Expression
  • Tags / Fusion Proteins
    • TEV cleavable site (ENLYFQG) (C terminal on backbone)
    • Twin-strep-tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert can be subcloned into XhoI site via Gibson Assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOET3-N-TEV-Twin-Strep was a gift from Chiaki Murakami (Addgene plasmid # 203323 ; http://n2t.net/addgene:203323 ; RRID:Addgene_203323)

  • For your References section:

    Diacylglycerol kinase zeta interacts with sphingomyelin synthase 1 and sphingomyelin synthase-related protein via different regions. Furuta M, Murakami C, Numagami Y, Suzuki R, Sakane F. FEBS Open Bio. 2023 May 11. doi: 10.1002/2211-5463.13628. 10.1002/2211-5463.13628 PubMed 37166445
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