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Addgene

hBCAM-M3
(Plasmid #203319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hBCAM/hMUC18
  • Species
    H. sapiens (human)
  • Mutation
    IgG V1-C1 of hMuc18 fused to Ig like C2-C3 of hBCAM
  • Tag / Fusion Protein
    • Flag (DYKDDDK) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Origene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A G341E mutation in the insert is present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hBCAM-M3 was a gift from William Hahn (Addgene plasmid # 203319 ; http://n2t.net/addgene:203319 ; RRID:Addgene_203319)

  • For your References section:

    Discovery of antibodies and cognate surface targets for ovarian cancer by surface profiling. Schrofelbauer B, Kimes PK, Hauke P, Reid CE, Shao K, Hill SJ, Irizarry R, Hahn WC. Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2206751120. doi: 10.1073/pnas.2206751120. Epub 2022 Dec 27. 10.1073/pnas.2206751120 PubMed 36574667
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