PlayBack-U6-Xba1
(Plasmid
#203300)
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Purpose(Empty Backbone) For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePlayBack Vector
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PlayBack-U6-Xba1 was a gift from Aleksandar Necakov (Addgene plasmid # 203300 ; http://n2t.net/addgene:203300 ; RRID:Addgene_203300) -
For your References section:
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs. Foran G, Hallam RD, Megaly M, Turgambayeva A, Necakov A. Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10. 10.2144/btn-2023-0042 PubMed 37815818