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Addgene

pV132-vCas9
(Plasmid #203223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX165
  • Total vector size (bp) 8214
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vCas9
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    4272
  • Mutation
    S55R R976A K1003A T1314R
  • Promoter CBh

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BshTI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CBh-FWD
  • 3′ sequencing primer bGH-REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pV132-vCas9 was a gift from Vikash Chauhan & Robert Langer & Phil Sharp (Addgene plasmid # 203223 ; http://n2t.net/addgene:203223 ; RRID:Addgene_203223)

  • For your References section:

    Altered DNA repair pathway engagement by engineered CRISPR-Cas9 nucleases. Chauhan VP, Sharp PA, Langer R. Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2300605120. doi: 10.1073/pnas.2300605120. Epub 2023 Mar 7. 10.1073/pnas.2300605120 PubMed 36881621
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