pTE-10M0X
(Plasmid #20318)

• Depositing Lab: Dirk Schnappinger
• Publication: Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

Backbone

• Vector backbone: pTE-mcs
• Backbone size w/o insert (bp): 5133
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Pimyc-tetR#10
• Species: synthetic gene derived from Tn10 tetR
• Insert Size (bp): 970

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.