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Addgene

pTE-10M0X
(Plasmid #20318)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTE-mcs
  • Backbone size w/o insert (bp) 5133
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Pimyc-tetR#10
  • Species
    synthetic gene derived from Tn10 tetR
  • Insert Size (bp)
    970

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE-10M0X was a gift from Dirk Schnappinger (Addgene plasmid # 20318 ; http://n2t.net/addgene:20318 ; RRID:Addgene_20318)

  • For your References section:

    Improved tetracycline repressors for gene silencing in mycobacteria. Klotzsche M, Ehrt S, Schnappinger D. Nucleic Acids Res. 2009 Jan 27. ():. 10.1093/nar/gkp015 PubMed 19174563
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