pKG-GW1-MaUPPS-A
(Plasmid
#203174)
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PurposeYeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKG-GW1
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameuppS
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Alt nameUPPS-A
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SpeciesMethanosarcina acetivorans
- Promoter TDH3
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CTACTTTTATAGTTAGTC
- 3′ sequencing primer ATAAATTGAAACTAAGTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKG-GW1-MaUPPS-A was a gift from William Sessa (Addgene plasmid # 203174 ; http://n2t.net/addgene:203174 ; RRID:Addgene_203174) -
For your References section:
A conserved C-terminal RXG motif in the NgBR subunit of cis-prenyltransferase is critical for prenyltransferase activity. Grabinska KA, Edani BH, Park EJ, Kraehling JR, Sessa WC. J Biol Chem. 2017 Oct 20;292(42):17351-17361. doi: 10.1074/jbc.M117.806034. Epub 2017 Aug 23. 10.1074/jbc.M117.806034 PubMed 28842490