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Addgene

pKGmDUET-NgBR/hCIT
(Plasmid #203173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    NUS1
  • Alt name
    NgBR
  • Species
    H. sapiens (human)
  • Entrez Gene
    NUS1 (a.k.a. C6orf68, CDG1AA, MGC:7199, MRD55, NgBR, TANGO14)
  • Promoter CMV
  • Tag / Fusion Protein
    • internally 6HIS tagged (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    DHDDS
  • Alt name
    hCIT
  • Species
    H. sapiens (human)
  • Entrez Gene
    DHDDS (a.k.a. CIT, CPT, DEDSM, DS, HDS, RP59, hCIT)
  • Tag / Fusion Protein
    • Strep-Tag II-TEV (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKGmDUET-NgBR/hCIT was a gift from William Sessa (Addgene plasmid # 203173 ; http://n2t.net/addgene:203173 ; RRID:Addgene_203173)

  • For your References section:

    A conserved C-terminal RXG motif in the NgBR subunit of cis-prenyltransferase is critical for prenyltransferase activity. Grabinska KA, Edani BH, Park EJ, Kraehling JR, Sessa WC. J Biol Chem. 2017 Oct 20;292(42):17351-17361. doi: 10.1074/jbc.M117.806034. Epub 2017 Aug 23. 10.1074/jbc.M117.806034 PubMed 28842490
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