pKG-GW1-SRT1
(Plasmid
#203171)
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PurposeYeast expression vector for yeast SRT1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKG-GW1
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSRT1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSRT1 (a.k.a. YMR101C)
- Promoter TDH3
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CTACTTTTATAGTTAGTC
- 3′ sequencing primer ATAAATTGAAACTAAGTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKG-GW1-SRT1 was a gift from William Sessa (Addgene plasmid # 203171 ; http://n2t.net/addgene:203171 ; RRID:Addgene_203171) -
For your References section:
Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation. Park EJ, Grabinska KA, Guan Z, Stranecky V, Hartmannova H, Hodanova K, Baresova V, Sovova J, Jozsef L, Ondruskova N, Hansikova H, Honzik T, Zeman J, Hulkova H, Wen R, Kmoch S, Sessa WC. Cell Metab. 2014 Sep 2;20(3):448-57. doi: 10.1016/j.cmet.2014.06.016. Epub 2014 Jul 24. 10.1016/j.cmet.2014.06.016 PubMed 25066056