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Addgene

pKG-GW2-NgBR
(Plasmid #203165)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKG-GW2
  • Vector type
    Yeast Expression
  • Selectable markers
    MET15

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NUS1
  • Alt name
    NgBR
  • Species
    H. sapiens (human)
  • Entrez Gene
    NUS1 (a.k.a. C6orf68, CDG1AA, MGC:7199, MRD55, NgBR, TANGO14)
  • Promoter TDH3

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CTACTTTTATAGTTAGTC
  • 3′ sequencing primer ATAAATTGAAACTAAGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG-GW2-NgBR was a gift from William Sessa (Addgene plasmid # 203165 ; http://n2t.net/addgene:203165 ; RRID:Addgene_203165)

  • For your References section:

    Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation. Park EJ, Grabinska KA, Guan Z, Stranecky V, Hartmannova H, Hodanova K, Baresova V, Sovova J, Jozsef L, Ondruskova N, Hansikova H, Honzik T, Zeman J, Hulkova H, Wen R, Kmoch S, Sessa WC. Cell Metab. 2014 Sep 2;20(3):448-57. doi: 10.1016/j.cmet.2014.06.016. Epub 2014 Jul 24. 10.1016/j.cmet.2014.06.016 PubMed 25066056
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