pTC-28S15-0X
(Plasmid #20316)

• Depositing Lab: Dirk Schnappinger
• Publication: Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

Backbone

• Vector backbone: pTC-mcs
• Backbone size w/o insert (bp): 3942
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Psmyc-tetR#28
• Species: synthetic gene derived from Tn10 tetR
• Insert Size (bp): 1152

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A missense mutation was detected in the integrase ORF (T3899A). The impact of this mutation on plasmid functionality is unknown.