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Addgene

pNZ18-CAH
(Plasmid #203159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSH71
  • Backbone size w/o insert (bp) 5703
  • Total vector size (bp) 7081
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    HIS3 ; Kanamycin/Neomycin (for Acholeplasma laidlawii)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli MC1061
  • Growth instructions
    Plasmid should be maintained in a RecA+ E. coli strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
  • Alt name
    CEN6, ARSH4, HIS3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1378

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.09.21.508766 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNZ18-CAH was a gift from Bogumil Karas (Addgene plasmid # 203159 ; http://n2t.net/addgene:203159 ; RRID:Addgene_203159)

  • For your References section:

    An Expanded Genetic Toolbox to Accelerate the Creation of Acholeplasma laidlawii Driven by Synthetic Genomes. Nucifora DP, Mehta ND, Giguere DJ, Karas BJ. ACS Synth Biol. 2024 Jan 19;13(1):45-53. doi: 10.1021/acssynbio.3c00399. Epub 2023 Dec 19. 10.1021/acssynbio.3c00399 PubMed 38113213
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