pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
(Plasmid #20295)

• Purpose: Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation
• Depositing Lab: Karl Deisseroth
• Publication: Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Backbone

• Vector backbone: pLenti-CaMKIIa
• Backbone size w/o insert (bp): 9250
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: channelrhodopsin-2
• Alt name: ChR2(C128T)
• Alt name: cop4
• Alt name: channelopsin-2
• Species: C. reinhardtii
• Insert Size (bp): 1662
• Mutation: changed Cysteine 128 to Threonine
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see http://www.optogenetics.org

for additional information.