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Addgene

Salsa-BC5-P1A5
(Plasmid #202846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCER206
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP/BC5
  • Species
    Synthetic
  • Insert Size (bp)
    730
  • Promoter Tet Response Element (TRE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Custom primer (GAAGCGCGATCACATGG)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Salsa-BC5-P1A5 was a gift from Noah Palm (Addgene plasmid # 202846 ; http://n2t.net/addgene:202846 ; RRID:Addgene_202846)

  • For your References section:

    Highly multiplexed bioactivity screening reveals human and microbiota metabolome-GPCRome interactions. Chen H, Rosen CE, Gonzalez-Hernandez JA, Song D, Potempa J, Ring AM, Palm NW. Cell. 2023 Jul 6;186(14):3095-3110.e19. doi: 10.1016/j.cell.2023.05.024. Epub 2023 Jun 14. 10.1016/j.cell.2023.05.024 PubMed 37321219
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