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Addgene

TetO-CDK5RAP2
(Plasmid #202840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified from Addgene 41152
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK5RAP2
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDK5RAP2 (a.k.a. C48, Cep215, MCPH3)
  • Promoter TetO

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PciI (unknown if destroyed)
  • 3′ cloning site AfeI (unknown if destroyed)
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetO-CDK5RAP2 was a gift from Matthew Anderson (Addgene plasmid # 202840 ; http://n2t.net/addgene:202840 ; RRID:Addgene_202840)

  • For your References section:

    Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson. Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. 10.1038/s42003-023-04683-8 PubMed 36997626
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