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Addgene

pLVX-SVA-lncRNA AK057321 shRNA 2S
(Plasmid #202838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 8995
  • Total vector size (bp) 9060
  • Modifications to backbone
    The CMV promoter in pLVX-shRNA2-mcherry was excised with BamHI and AgeI digestion and replaced with the UbC promoter, P2A, the zeomycin resistance gene and T2A in frame with mcherry using NEBuilder HiFi DNA Assembly (NEB). UbC, P2A, and T2A sequences were PCR-amplified from pLV-TetO-hNGN2-eGFP-Puro (Addgene #79823) and the zeocin resistance gene sequence was amplified by PCR from FRT-Em7-Zeo-FRT plasmid (Thermo Fisher).
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pLVX-SVA-lncRNA shRNA 2S
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA 2S target sequence: 5'-CATCAAGATGCAGTCTCCC-3'
Use in combination with pLVX-SVA-lncRNA AK057321 1A (202834) and pLVX-SVA-lncRNA AK057321 1S (202836) for best knockdown of SVA-lncRNA AK057321.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-SVA-lncRNA AK057321 shRNA 2S was a gift from Matthew Anderson (Addgene plasmid # 202838 ; http://n2t.net/addgene:202838 ; RRID:Addgene_202838)

  • For your References section:

    Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson. Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. 10.1038/s42003-023-04683-8 PubMed 36997626
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