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pGL3-luciferase-reporter-AGPAT3-SVA
(Plasmid #202827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4996
  • Total vector size (bp) 6530
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    intronic SVA located in AGPAT3 gene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1534
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-luciferase-reporter-AGPAT3-SVA was a gift from Matthew Anderson (Addgene plasmid # 202827 ; http://n2t.net/addgene:202827 ; RRID:Addgene_202827)

  • For your References section:

    Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson. Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. 10.1038/s42003-023-04683-8 PubMed 36997626
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