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Addgene

lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP
(Plasmid #202825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified version of 98291
  • Backbone size w/o insert (bp) 12790
  • Total vector size (bp) 13651
  • Modifications to backbone
    This vector was modified from Addgene 98291. The hygromycin resistance gene was excised by digestion with PshAI and MluI. T2A and blue fluorescent protein (BFP) were inserted in frame after the MluI site. Two sgRNAs were then cloned in tandem, each with their U6 promoter into digested lentiCRISPRv2-BFP vector (KpnI and NheI) using NEBuilder HiFi DNA Assembly(NEB).
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    862
  • Promoter U6 - two

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP was a gift from Matthew Anderson (Addgene plasmid # 202825 ; http://n2t.net/addgene:202825 ; RRID:Addgene_202825)

  • For your References section:

    Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson. Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. 10.1038/s42003-023-04683-8 PubMed 36997626
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