lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry
(Plasmid #202822)

• Purpose: Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression
• Depositing Lab: Matthew Anderson
• Publication: Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.

Backbone

• Vector backbone: modified version of 98291
• Backbone size w/o insert (bp): 12817
• Total vector size (bp): 13679
• Modifications to backbone: This vector was modified from Addgene 98291. The hygromycin resistance gene was excised by digestion with PshAI and MluI and replaced with PCR-amplified mcherry. lentiCRISPRv2-mcherry was then digested with KpnI and NheI and PCR fragments containing two sgRNAs, each with their own U6 promoter were assembled with NEBuilder HiFi DNA Assembly(NEB).
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: two sgRNAs that delete exon 1 within the ZNF91 gene
• Species: H. sapiens (human)
• Promoter: U6 - two

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: whole plasmid sequencing
• 3′ sequencing primer: whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sgRNAs to delete exon 1 within ZNF91 were published in:
https://pubmed.ncbi.nlm.nih.gov/33722937/