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pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry
(Plasmid #202818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 7844
  • Total vector size (bp) 9696
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SVA-lncRNA AK057321
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1763
  • Mutation
    addition of a dual aptamer RNA tag 5' to the start of SVA-lncRNA AK057321 sequence
  • GenBank ID
    AK057321.1
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SVA-lncRNA AK057321, Homo sapiens cDNA FLJ32759 fis, clone TESTI2001793

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Walker et al. for description of RNA dual aptamer tags, https://pubmed.ncbi.nlm.nih.gov/21431756

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry was a gift from Matthew Anderson (Addgene plasmid # 202818 ; http://n2t.net/addgene:202818 ; RRID:Addgene_202818)

  • For your References section:

    Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson. Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. 10.1038/s42003-023-04683-8 PubMed 36997626
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