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Addgene

pHYX173
(Plasmid #202817)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202817 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHYX137 and pHYX138
  • Backbone size w/o insert (bp) 7134
  • Total vector size (bp) 7845
  • Modifications to backbone
    The TEV protease gene was cloned into pHYX137 linearized by EcoRV. The resulting plasmid was then digested by SwaI. The pHYX138 was digested by PmeI. Both restriction reactions were then mixed and transformed into E. coli for in vivo assembly to give pHYX173.
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEV protease
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter ScPCK1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYX173 was a gift from Jean-Félix Dallery (Addgene plasmid # 202817 ; http://n2t.net/addgene:202817 ; RRID:Addgene_202817)

  • For your References section:

    Yeast-based heterologous production of the colletochlorin family of fungal secondary metabolites. Geistodt-Kiener A, Totozafy JC, Le Goff G, Vergne J, Sakai K, Ouazzani J, Mouille G, Viaud M, O'Connell RJ, Dallery JF. Metab Eng. 2023 Oct 18:S1096-7176(23)00142-8. doi: 10.1016/j.ymben.2023.10.002. 10.1016/j.ymben.2023.10.002 PubMed 37863177
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