Skip to main content
Addgene

pHYX143
(Plasmid #202816)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202816 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHYX137
  • Backbone size w/o insert (bp) 7779
  • Total vector size (bp) 8475
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet-I
  • Species
    Synthetic
  • Insert Size (bp)
    696
  • GenBank ID
    APD76536.1 KY021424.1
  • Promoter ScPCK1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCTGAAGAAGGAGCTCGCTC
  • 3′ sequencing primer GGATCAGCTCGGCCTTGTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYX143 was a gift from Jean-Félix Dallery (Addgene plasmid # 202816 ; http://n2t.net/addgene:202816 ; RRID:Addgene_202816)

  • For your References section:

    Yeast-based heterologous production of the colletochlorin family of fungal secondary metabolites. Geistodt-Kiener A, Totozafy JC, Le Goff G, Vergne J, Sakai K, Ouazzani J, Mouille G, Viaud M, O'Connell RJ, Dallery JF. Metab Eng. 2023 Oct 18:S1096-7176(23)00142-8. doi: 10.1016/j.ymben.2023.10.002. 10.1016/j.ymben.2023.10.002 PubMed 37863177
Related items: