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Addgene

pHYX137
(Plasmid #202814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pV2A-T
  • Backbone size (bp) 7779
  • Modifications to backbone
    Insertion of 2µ origin of replication, LEU2 selection, PCK1 promoter and PRM9 terminator from Saccharomyces cerevisiae. TetON promoter and TrpC terminator removed.
  • Vector type
    Yeast Expression
  • Promoter ScPCK1
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYX137 was a gift from Jean-Félix Dallery (Addgene plasmid # 202814 ; http://n2t.net/addgene:202814 ; RRID:Addgene_202814)

  • For your References section:

    Yeast-based heterologous production of the colletochlorin family of fungal secondary metabolites. Geistodt-Kiener A, Totozafy JC, Le Goff G, Vergne J, Sakai K, Ouazzani J, Mouille G, Viaud M, O'Connell RJ, Dallery JF. Metab Eng. 2023 Oct 18:S1096-7176(23)00142-8. doi: 10.1016/j.ymben.2023.10.002. 10.1016/j.ymben.2023.10.002 PubMed 37863177
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