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pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro
(Plasmid #202800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EFalpha-mcherry-puro
  • Backbone manufacturer
    purchased from Biosettia
  • Backbone size w/o insert (bp) 9472
  • Total vector size (bp) 11235
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SVA-lncRNA AK057321
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1763
  • GenBank ID
    AK057321.1
  • Promoter EFalpha (with intron)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Homo sapiens cDNA FLJ32759 fis, clone TESTI2001793

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro was a gift from Matthew Anderson (Addgene plasmid # 202800 ; http://n2t.net/addgene:202800 ; RRID:Addgene_202800)

  • For your References section:

    Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson. Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. 10.1038/s42003-023-04683-8 PubMed 36997626
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