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Addgene

CD-MPR-GFP-RUSH
(Plasmid #202797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Original RUSH construct (gift of F. Perezand G. Boncompain, Curie Institute; Boncompain et al., 2012, Nature Methods)
  • Total vector size (bp) 6721
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    M6PR
  • Alt name
    CD-MPR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    756
  • Mutation
    Changed 40 K (AAA) with R (AGA)
  • GenBank ID
    NM_002355.4
  • Entrez Gene
    M6PR (a.k.a. CD-M6PR, CD-MPR, MPR 46, MPR-46, MPR46, SMPR)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD-MPR-GFP-RUSH was a gift from Juan Bonifacino (Addgene plasmid # 202797 ; http://n2t.net/addgene:202797 ; RRID:Addgene_202797)

  • For your References section:

    Segregation in the Golgi complex precedes export of endolysosomal proteins in distinct transport carriers. Chen Y, Gershlick DC, Park SY, Bonifacino JS. J Cell Biol. 2017 Dec 4;216(12):4141-4151. doi: 10.1083/jcb.201707172. Epub 2017 Oct 4. 10.1083/jcb.201707172 PubMed 28978644
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