pET28a-Hisx6-Sirt6-S56A
(Plasmid
#20278)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSirt6
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SpeciesM. musculus (mouse)
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Insert Size (bp)1004
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MutationSerine 56 to Alanine
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GenBank IDNM_181586
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Entrez GeneSirt6 (a.k.a. 2810449N18Rik, Sir2l6, mSIRT6)
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Tag
/ Fusion Protein
- Hisx6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-Hisx6-Sirt6-S56A was a gift from Leonard Guarente (Addgene plasmid # 20278 ; http://n2t.net/addgene:20278 ; RRID:Addgene_20278) -
For your References section:
Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase. Liszt G, Ford E, Kurtev M, Guarente L. J Biol Chem. 2005 Jun 3. 280(22):21313-20. 10.1074/jbc.M413296200 PubMed 15795229