pDGE1176
(Plasmid
#202774)
-
PurposeCRISPR-KO in Arabidopsis thaliana
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepJOG272
-
Vector typePlant Expression, CRISPR
-
Selectable markersRED seed fluorescence (FAST)
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Growth instructionsStrain may grow fairly slow. Use clean glassware or (better) single use plastic culture tubes.
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert nameFAST marker
Gene/Insert 2
-
Gene/Insert namepRPS5a:zCas9_tNbEU
Gene/Insert 3
-
Gene/Insert nameccdB cassette (BsaI-excisable)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDGE1176 was a gift from Johannes Stuttmann (Addgene plasmid # 202774 ; http://n2t.net/addgene:202774 ; RRID:Addgene_202774) -
For your References section:
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two. Ordon J, Kiel N, Becker D, Kretschmer C, Schulze-Lefert P, Stuttmann J. Plant Methods. 2023 Mar 28;19(1):30. doi: 10.1186/s13007-023-01010-4. 10.1186/s13007-023-01010-4 PubMed 36978193
