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Addgene

pDGE1176
(Plasmid #202774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJOG272
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    RED seed fluorescence (FAST)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Strain may grow fairly slow. Use clean glassware or (better) single use plastic culture tubes.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    FAST marker

Gene/Insert 2

  • Gene/Insert name
    pRPS5a:zCas9_tNbEU

Gene/Insert 3

  • Gene/Insert name
    ccdB cassette (BsaI-excisable)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGE1176 was a gift from Johannes Stuttmann (Addgene plasmid # 202774 ; http://n2t.net/addgene:202774 ; RRID:Addgene_202774)

  • For your References section:

    Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two. Ordon J, Kiel N, Becker D, Kretschmer C, Schulze-Lefert P, Stuttmann J. Plant Methods. 2023 Mar 28;19(1):30. doi: 10.1186/s13007-023-01010-4. 10.1186/s13007-023-01010-4 PubMed 36978193
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