pCMV-CMP-PE-V1
(Plasmid
#202769)
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PurposeExpresses CMP-PE-V1 in mammalian cells for prime editing
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 3399
- Total vector size (bp) 10326
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMP-PE-V1
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SpeciesSynthetic
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Insert Size (bp)6927
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-CMP-PE-V1 was a gift from Kyoungmi Kim (Addgene plasmid # 202769 ; http://n2t.net/addgene:202769 ; RRID:Addgene_202769) -
For your References section:
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor. Park SJ, Jeong TY, Shin SK, Yoon DE, Lim SY, Kim SP, Choi J, Lee H, Hong JI, Ahn J, Seong JK, Kim K. Genome Biol. 2021 Jun 3;22(1):170. doi: 10.1186/s13059-021-02389-w. 10.1186/s13059-021-02389-w PubMed 34082781
