HSA3_His
(Plasmid #202765)

• Purpose: human serum albumin design HSA3, in pET29b with C-terminal 6His-tag
• Depositing Lab: Sarel Fleishman
• Publication: Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. doi: 10.1016/j.jmb.2023.168191. Epub 2023 Jun 28.

Backbone

• Vector backbone: pET29b
• Total vector size (bp): 6996
• Modifications to backbone: NdeI restriction site was changed to NcoI
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HSA3
• Species: Synthetic
• Insert Size (bp): 1764
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • HSA3_pET29b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry