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Addgene

TLCV2 - sgAP2S1 #2
(Plasmid #202757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TLCV2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA targeting AP2S1
  • gRNA/shRNA sequence
    CAAACACACCAACTTTGTGG
  • Species
    H. sapiens (human)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TLCV2 - sgAP2S1 #2 was a gift from Christopher Counter (Addgene plasmid # 202757 ; http://n2t.net/addgene:202757 ; RRID:Addgene_202757)

  • For your References section:

    The essential clathrin adapter protein complex-2 is tumor suppressive specifically in vivo. Zimmerman SP, DeGraw LB, Counter CM. Nat Commun. 2025 Mar 6;16(1):2254. doi: 10.1038/s41467-025-57521-2. 10.1038/s41467-025-57521-2 PubMed 40050266
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