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Addgene

PH-PLCd1-mNGx2
(Plasmid #202752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmNG2-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLCD1v2(1-170): mNG2:mNG2
  • Species
    H. sapiens (human)
  • Entrez Gene
    PLCD1 (a.k.a. NDNC3, PLC-III)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PH-PLCd1-mNGx2 was a gift from Gerry Hammond (Addgene plasmid # 202752 ; http://n2t.net/addgene:202752 ; RRID:Addgene_202752)

  • For your References section:

    A novel homeostatic mechanism tunes PI(4,5)P2-dependent signaling at the plasma membrane. Wills RC, Doyle CP, Zewe JP, Pacheco J, Hansen SD, Hammond GRV. J Cell Sci. 2023 Aug 3:jcs.261494. doi: 10.1242/jcs.261494. 10.1242/jcs.261494 PubMed 37534432
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