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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSirt6
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SpeciesM. musculus (mouse)
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Insert Size (bp)1004
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GenBank IDNM_181586
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Entrez GeneSirt6 (a.k.a. 2810449N18Rik, Sir2l6, mSIRT6)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSirt6-GFP was a gift from Leonard Guarente (Addgene plasmid # 20275 ; http://n2t.net/addgene:20275 ; RRID:Addgene_20275) -
For your References section:
Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase. Liszt G, Ford E, Kurtev M, Guarente L. J Biol Chem. 2005 Jun 3. 280(22):21313-20. 10.1074/jbc.M413296200 PubMed 15795229
