pTKEI-Dest-loxP-sfGFP
(Plasmid
#202715)
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PurposeEmpty expression vector containing Cre recombinase reporter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTKEI-Dest
- Backbone size w/o insert (bp) 4305
- Total vector size (bp) 5579
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePw7-loxP-term-loxP-sfGFP
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SpeciesSynthetic
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Insert Size (bp)1049
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://pubmed.ncbi.nlm.nih.gov/37293111/ for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTKEI-Dest-loxP-sfGFP was a gift from Mary Dunlop (Addgene plasmid # 202715 ; http://n2t.net/addgene:202715 ; RRID:Addgene_202715) -
For your References section:
Comprehensive Screening of a Light-Inducible Split Cre Recombinase with Domain Insertion Profiling. Tague N, Andreani V, Fan Y, Timp W, Dunlop MJ. ACS Synth Biol. 2023 Oct 3. doi: 10.1021/acssynbio.3c00328. 10.1021/acssynbio.3c00328 PubMed 37788288