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pCMV-FLAG-SIRT7-S112A
(Plasmid #20269)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20269 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV-Tag-4a
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIRT7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1232
  • Mutation
    Serine 112 to Alanine
  • GenBank ID
    NM_016528
  • Entrez Gene
    SIRT7 (a.k.a. SIR2L7)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-FLAG-SIRT7-S112A was a gift from Leonard Guarente (Addgene plasmid # 20269)

  • For your References section:

    Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription. Ford E, Voit R, Liszt G, Magin C, Grummt I, Guarente L. Genes Dev. 2006 May 1. 20(9):1075-80. 10.1101/gad.1399706 PubMed 16618798
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