PAmCherry1-N1-Lyn11
(Plasmid
#202683)
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PurposePhotoactivatable lipid biosensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmEos2-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLYN(1-11):PAmCherry1
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SpeciesH. sapiens (human)
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Entrez GeneLYN (a.k.a. JTK8, SAIDV, p53Lyn, p56Lyn)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAmCherry1-N1-Lyn11 was a gift from Gerry Hammond (Addgene plasmid # 202683 ; http://n2t.net/addgene:202683 ; RRID:Addgene_202683) -
For your References section:
PI(4,5)P2 diffuses freely in the plasma membrane even within high-density effector protein complexes. Pacheco J, Cassidy AC, Zewe JP, Wills RC, Hammond GRV. J Cell Biol. 2023 Feb 6;222(2):e202204099. doi: 10.1083/jcb.202204099. Epub 2022 Nov 23. 10.1083/jcb.202204099 PubMed 36416724