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Addgene

PAmCherry1-N1-Tubbyc
(Plasmid #202677)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202677 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPAmCherry-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus Tub (243-505): PAmCherry1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tub (a.k.a. rd5, tub)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PAmCherry1-N1-Tubbyc was a gift from Gerry Hammond (Addgene plasmid # 202677 ; http://n2t.net/addgene:202677 ; RRID:Addgene_202677)

  • For your References section:

    PI(4,5)P2 diffuses freely in the plasma membrane even within high-density effector protein complexes. Pacheco J, Cassidy AC, Zewe JP, Wills RC, Hammond GRV. J Cell Biol. 2023 Feb 6;222(2):e202204099. doi: 10.1083/jcb.202204099. Epub 2022 Nov 23. 10.1083/jcb.202204099 PubMed 36416724
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