pLVX-TetOne-Puro-PSMD4
(Plasmid
#202666)
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PurposeTet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVx-TetOne-Puro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameproteasome 26S subunit ubiquitin receptor, non-ATPase 4
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SpeciesH. sapiens (human)
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Entrez GenePSMD4 (a.k.a. AF, AF-1, ASF, MCB1, Rpn10, S5A, pUB-R5)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-TetOne-Puro-PSMD4 was a gift from Jason Sheltzer (Addgene plasmid # 202666 ; http://n2t.net/addgene:202666 ; RRID:Addgene_202666)