pLVX-TetOne-Puro-MCL1
(Plasmid #202663)

• Purpose: Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette
• Depositing Lab: Jason Sheltzer
• Publication: Oncogene-like addiction to aneuploidy in human cancers (unpublished)

Backbone

• Vector backbone: pLVx-TetOne-Puro
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MCL1 apoptosis regulator, BCL2 family member
• Species: H. sapiens (human)
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pLVX TetOne Puro MCL1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry