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Silene_vulgaris _TyrRS_MitoTP_pK7FWG2
(Plasmid #202658)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202658 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7FWG2
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria
  • Species
    Silene vulgaris
  • Tag / Fusion Protein
    • Green Florescent Protien (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Silene_vulgaris _TyrRS_MitoTP_pK7FWG2 was a gift from Daniel Sloan (Addgene plasmid # 202658 ; http://n2t.net/addgene:202658 ; RRID:Addgene_202658)

  • For your References section:

    Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions in Plants With Extensive Mitochondrial tRNA Gene Loss. Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, Sloan DB. Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 10.1093/molbev/msad163. 10.1093/molbev/msad163 PubMed 37463427
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