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Addgene

Shim_MetRS_TP_GFP_pK7FWG2
(Plasmid #202650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7FWG2
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-terminal transit peptide from putative cytosolic MetRS
  • Species
    Sapria himilayana
  • Tag / Fusion Protein
    • Green Florescent Protien (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Shim_MetRS_TP_GFP_pK7FWG2 was a gift from Daniel Sloan (Addgene plasmid # 202650 ; http://n2t.net/addgene:202650 ; RRID:Addgene_202650)

  • For your References section:

    Photosynthesis drives retention of bacterial-like tRNA metabolism in plant organelles. DeTar RA, Chustecki J, Martinez-Hottovy A, Cerrioti LF, Broz AK, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB. bioRxiv 2023.08.01.551541 10.1101/2023.08.01.551541
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