Skip to main content
Addgene

pSELIS-RamR
(Plasmid #202631)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSELIS
  • Total vector size (bp) 5966
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sh ble
  • Species
    Synthetic
  • Insert Size (bp)
    375
  • GenBank ID
    CAA37050.1
  • Promoter Lambda promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGACGGTTATTGGAGGAGGTTgacat
  • 3′ sequencing primer ggattaataatctggctttttatattctctcttta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSELIS-RamR was a gift from Andrew Ellington (Addgene plasmid # 202631 ; http://n2t.net/addgene:202631 ; RRID:Addgene_202631)

  • For your References section:

    Using fungible biosensors to evolve improved alkaloid biosyntheses. d'Oelsnitz S, Kim W, Burkholder NT, Javanmardi K, Thyer R, Zhang Y, Alper HS, Ellington AD. Nat Chem Biol. 2022 Jul 7. pii: 10.1038/s41589-022-01072-w. doi: 10.1038/s41589-022-01072-w. 10.1038/s41589-022-01072-w PubMed 35799063
Related items:
Commonly requested with: