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Addgene

gGBEv6.3-SpG
(Plasmid #202630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nSpG(D10A)-MPGv6.3
  • Species
    Synthetic
  • Mutation
    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R; MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202A, G203A, S206A, K210A, Q294R
  • Promoter hU6, EF1a, CBh

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gGBEv6.3-SpG was a gift from Huawei Tong (Addgene plasmid # 202630 ; http://n2t.net/addgene:202630 ; RRID:Addgene_202630)

  • For your References section:

    Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase. Tong H, Liu N, Wei Y, Zhou Y, Li Y, Wu D, Jin M, Cui S, Li H, Li G, Zhou J, Yuan Y, Zhang H, Shi L, Yao X, Yang H. Natl Sci Rev. 2023 May 16;10(8):nwad143. doi: 10.1093/nsr/nwad143. eCollection 2023 Aug. 10.1093/nsr/nwad143 PubMed 37404457
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