gGBEv6.3-SpCas9
(Plasmid
#202629)
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Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF1a
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenSpCas9(D10A)-MPGv6.3
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SpeciesSynthetic
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MutationMPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202A, G203A, S206A, K210A, Q294R
- Promoter hU6, EF1a, CBh
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gGBEv6.3-SpCas9 was a gift from Huawei Tong (Addgene plasmid # 202629 ; http://n2t.net/addgene:202629 ; RRID:Addgene_202629) -
For your References section:
Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase. Tong H, Liu N, Wei Y, Zhou Y, Li Y, Wu D, Jin M, Cui S, Li H, Li G, Zhou J, Yuan Y, Zhang H, Shi L, Yao X, Yang H. Natl Sci Rev. 2023 May 16;10(8):nwad143. doi: 10.1093/nsr/nwad143. eCollection 2023 Aug. 10.1093/nsr/nwad143 PubMed 37404457
