pJB158
(Plasmid
#202601)
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Purpose(Empty Backbone) RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFE604T
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Vector typeBacterial Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EC100D pir+
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Growth instructionsVector has R6K origin of replication for pir+ strains and F-plasmid origin of replication for pir- strains
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Copy numberLow Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJB158 was a gift from Athanasios Typas (Addgene plasmid # 202601 ; http://n2t.net/addgene:202601 ; RRID:Addgene_202601) -
For your References section:
TAC-TIC, a high-throughput genetics method to identify triggers or blockers of bacterial toxin-antitoxin systems. Bobonis J, Yang ALJ, Voogdt CGP, Typas A. Nat Protoc. 2024 May 9. doi: 10.1038/s41596-024-00988-y. 10.1038/s41596-024-00988-y PubMed 38724726
